Journal: Nanomedicine (London, England)

Article Title: Inorganic nanoparticles as potential regulators of immune response in dendritic cells.

doi: 10.2217/nnm-2017-0061

Figure Lengend Snippet: Figure 2. Protein corona formation. (A & B) Representative DLS curves acquired for (A) AuNPs and (B) SiO2NPs in before (orange for AuNPs and gray for SiO2NPs lines and empty circles) and incubated with Hep G2 (green lines and filled triangles) and A2780 (blue lines and filled circles) cell lysates; (C & D) CD spectra registered for (C) bare Hep G2 cell lysate (green line and empty triangles) and Hep G2 lysate-coated SiO2NPs@PC (gray line and empty squares), AuNPs@PC (yellow line and empty circles); (D) bare A2780 lysate (blue line and empty triangles) and A2780 lysate-coated SiO2NPs@PC (gray line and empty squares), AuNPs@PC (orange line and empty circles). AuNP: Gold nanoparticle; CD: Circular dichroism; DLS: Dynamic light scattering; NP: Nanoparticle; PC: Protein corona; SiO2NP: Silica nanoparticle.

Article Snippet: Whole cell lysates Hep G2 whole cell lysate was purchased from Novus Biologicals (CO, USA) and employed for DLS, CD and mass spectrometry; A2780 ovary carcinoma lysate was gently provided by Prof. Banci’s group (CERM, Florence, Italy) and employed for DLS, CD and mass spectrometry experiments, as well as for assays in the interaction between NPs@PC and DC; HCT-8 colon carcinoma lysate was obtained by HCT-8 colon carcinoma cell line kindly provided by Marcella Coronnello, (University of Florence).

Techniques: Incubation, Circular Dichroism

Journal: Endocrinology

Article Title: Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export.

doi: 10.1210/en.2015-1215

Figure Lengend Snippet: Figure 1. Endogenous Nur77 is knocked down using two different siRNA oligos and is detectable in MAC-T cells with a commercially available antibody. Cells were transfected with scramble (SCR) or Nur77 siRNA (Oligo No. 1 or Oligo No. 2). Total RNA (A) or protein (B) was isolated from WCLs after 48 h and analyzed for Nur77 by RT-qPCR (corrected for cyclophilin) or Western immunoblot (50 g protein), respectively. For the immunoblot, HSP60 served as a loading control. Bars represent mean SE of three experiments. C, To confirm specificity of the Nur77 antibody, MAC-T WCL (50 g), WCL from MAC-T cells transfected with myc-tagged Nur77 (3 g), HepG2 WCL (50 g), and nuclear fractions from MAC-T cells (5 g) were immunoblotted for Nur77 on one half of the membrane and myc on the other half. Actin served as a loading control.

Article Snippet: HepG2 cell lysate was obtained from Novus Biologicals.

Techniques: Transfection, Isolation, Quantitative RT-PCR, Western Blot, Control, Membrane